منابع مشابه
Purification and Biochemical Characterisation of Ricin from Castor Seeds
Ricin is a highly toxic plant toxin of Ricinus comtnunis seeds, commonly known as castor seeds. The toxin was extracted and purified using affinity and size exclusion chromatography. The purity of ricin was evaluated by the sodium dodecylsulphate-polyacrylamide gel electrophoresis. Purified ricin gives a single band under non-reduced condition and two bands under reduced condition. The molecula...
متن کاملStudies on Ricinus Lipase Enzyme Isolated from Castor Seeds
Healthy and bold seeds of castor variety “Aruna” were collected. The de-hulled castor kernels were separated from seed coat and finely grounded in an electric grinder. The powdered kernels were passed through 60 mesh sieve and defatted using n-hexane in a soxhlet extractor. After complete extraction of oil seed meal was made free from solvent at low temperature. The defatted seed meal was pulve...
متن کاملSynthesis of the crystalloid protein complex in vivo in the endosperm of developing castor bean seeds.
The synthesis of subunit polypeptides of the crystalloid protein complex has been examined in endosperm from developing castor bean (Ricinus communis L. cv Hale) seeds. Pulse-label and -chase studies in vivo have shown that synthesis initially involves the formation of high molecular weight precursors (50 to 60 kilodaltons) comprising peptide-linked acidic and basic polypeptides. Precursor proc...
متن کاملIn vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase isoforms in endosperm of developing castor oil seeds.
Our previous research characterized two phosphoenolpyruvate (PEP) carboxylase (PEPC) isoforms (PEPC1 and PEPC2) from developing castor oil seeds (COS). The association of a shared 107-kD subunit (p107) with an immunologically unrelated bacterial PEPC-type 64-kD polypeptide (p64) leads to marked physical and kinetic differences between the PEPC1 p107 homotetramer and PEPC2 p107/p64 heterooctamer...
متن کاملOn the Development of the Ribosomal System in the Endosperm of Germinating Castor Bean Seeds.
T'lhe important \ork of ileevers and collal)orators oni the metabolic evolution of the castor bean endosi)eri (lenlolnstrates the fitles.s of this imiaterial for the stu(lv of the lbiochemical activation (lurinig the earlxI p)hase of seed germiniationl. As shlown 1y these aithors. 1)oth respiration ii, vixo aind the oxi(lative activitv of miiitoclhoiudIrial plreparations markedly inicrease (lur...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1975
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.39.745